Protease Inhibitor:Article Title: TIF1γ inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BamHI New England Biolabs Cat#R0136L HindIII New England Biolabs Cat#R0104L EcoRI New England Biolabs Cat#R0101L XbaI New England Biolabs Cat#R0145L T4 DNA ligase buffer Thermo Fisher Scientific Cat#46300018 Lipofectamine 3000 Invitrogen Cat#L3000015 Puromycin Solarbio Cat#P8230 RIPA buffer Thermo Fisher Scientific Cat#89900 Protease inhibitor cocktail Sangon Biotech Cat#C600387 Phosphatase inhibitor cocktail Sangon Biotech Cat#C500017 Native sample loading buffer Sangon Biotech Cat#C506032 DTT Sangon Biotech Cat#B645939 TRIzol Thermo Fisher Scientific Cat#15596018 M-MLV (H-) Reverse Transcriptase Vazyme Cat#R021-01 Act-D Sigma-Aldrich Cat#129935 Nonidet P-40 Yeasen Cat#20103ES60 Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 Magnetic beads carrying antibodies against Flag Bimake Cat#B26102 Magnetic beads carrying antibodies against HA Bimake Cat#B26202 40,6-diamidino-2-phenylindole (DAPI) Beyotime Cat#P0131 Matrigel Corning Cat#354230 Human IL6 MESGEN Cat#MIL1006 Critical commercial assays Enhanced Chemiluminescence (ECL) kit Fdbio science Cat#FB8020 Silver Staining Kit CWBIO Cat#CW2012 ChIP-IT Express Kit Active Motif Cat#53008 Human IL6 ELISA Kit Beyotime Cat#PI330 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Deposited data RNA-seq This paper GSE210093 Experimental models: Cell lines Human HBE cells Procell Life Science & Technology company Cat#CL-0346 Human BEAS-2B cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-5067 Human A549 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-503 Human H1650 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-592 Human H1299 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-589 Human embryonic kidney (HEK) 293T cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-502 Experimental models: Organisms/strains Mouse: female BALB/c nude mice the Laboratory Animal Center of Soochow University N/A (Continued on next page) Cell Reports 41, 111513, October 18, 2022 e2 Article ll OPEN ACCESS
Reverse Transcription:Article Title: TIF1γ inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BamHI New England Biolabs Cat#R0136L HindIII New England Biolabs Cat#R0104L EcoRI New England Biolabs Cat#R0101L XbaI New England Biolabs Cat#R0145L T4 DNA ligase buffer Thermo Fisher Scientific Cat#46300018 Lipofectamine 3000 Invitrogen Cat#L3000015 Puromycin Solarbio Cat#P8230 RIPA buffer Thermo Fisher Scientific Cat#89900 Protease inhibitor cocktail Sangon Biotech Cat#C600387 Phosphatase inhibitor cocktail Sangon Biotech Cat#C500017 Native sample loading buffer Sangon Biotech Cat#C506032 DTT Sangon Biotech Cat#B645939 TRIzol Thermo Fisher Scientific Cat#15596018 M-MLV (H-) Reverse Transcriptase Vazyme Cat#R021-01 Act-D Sigma-Aldrich Cat#129935 Nonidet P-40 Yeasen Cat#20103ES60 Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 Magnetic beads carrying antibodies against Flag Bimake Cat#B26102 Magnetic beads carrying antibodies against HA Bimake Cat#B26202 40,6-diamidino-2-phenylindole (DAPI) Beyotime Cat#P0131 Matrigel Corning Cat#354230 Human IL6 MESGEN Cat#MIL1006 Critical commercial assays Enhanced Chemiluminescence (ECL) kit Fdbio science Cat#FB8020 Silver Staining Kit CWBIO Cat#CW2012 ChIP-IT Express Kit Active Motif Cat#53008 Human IL6 ELISA Kit Beyotime Cat#PI330 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Deposited data RNA-seq This paper GSE210093 Experimental models: Cell lines Human HBE cells Procell Life Science & Technology company Cat#CL-0346 Human BEAS-2B cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-5067 Human A549 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-503 Human H1650 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-592 Human H1299 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-589 Human embryonic kidney (HEK) 293T cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-502 Experimental models: Organisms/strains Mouse: female BALB/c nude mice the Laboratory Animal Center of Soochow University N/A (Continued on next page) Cell Reports 41, 111513, October 18, 2022 e2 Article ll OPEN ACCESS
Magnetic Beads:Article Title: TIF1γ inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BamHI New England Biolabs Cat#R0136L HindIII New England Biolabs Cat#R0104L EcoRI New England Biolabs Cat#R0101L XbaI New England Biolabs Cat#R0145L T4 DNA ligase buffer Thermo Fisher Scientific Cat#46300018 Lipofectamine 3000 Invitrogen Cat#L3000015 Puromycin Solarbio Cat#P8230 RIPA buffer Thermo Fisher Scientific Cat#89900 Protease inhibitor cocktail Sangon Biotech Cat#C600387 Phosphatase inhibitor cocktail Sangon Biotech Cat#C500017 Native sample loading buffer Sangon Biotech Cat#C506032 DTT Sangon Biotech Cat#B645939 TRIzol Thermo Fisher Scientific Cat#15596018 M-MLV (H-) Reverse Transcriptase Vazyme Cat#R021-01 Act-D Sigma-Aldrich Cat#129935 Nonidet P-40 Yeasen Cat#20103ES60 Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 Magnetic beads carrying antibodies against Flag Bimake Cat#B26102 Magnetic beads carrying antibodies against HA Bimake Cat#B26202 40,6-diamidino-2-phenylindole (DAPI) Beyotime Cat#P0131 Matrigel Corning Cat#354230 Human IL6 MESGEN Cat#MIL1006 Critical commercial assays Enhanced Chemiluminescence (ECL) kit Fdbio science Cat#FB8020 Silver Staining Kit CWBIO Cat#CW2012 ChIP-IT Express Kit Active Motif Cat#53008 Human IL6 ELISA Kit Beyotime Cat#PI330 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Deposited data RNA-seq This paper GSE210093 Experimental models: Cell lines Human HBE cells Procell Life Science & Technology company Cat#CL-0346 Human BEAS-2B cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-5067 Human A549 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-503 Human H1650 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-592 Human H1299 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-589 Human embryonic kidney (HEK) 293T cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-502 Experimental models: Organisms/strains Mouse: female BALB/c nude mice the Laboratory Animal Center of Soochow University N/A (Continued on next page) Cell Reports 41, 111513, October 18, 2022 e2 Article ll OPEN ACCESS
Silver Staining:Article Title: TIF1γ inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BamHI New England Biolabs Cat#R0136L HindIII New England Biolabs Cat#R0104L EcoRI New England Biolabs Cat#R0101L XbaI New England Biolabs Cat#R0145L T4 DNA ligase buffer Thermo Fisher Scientific Cat#46300018 Lipofectamine 3000 Invitrogen Cat#L3000015 Puromycin Solarbio Cat#P8230 RIPA buffer Thermo Fisher Scientific Cat#89900 Protease inhibitor cocktail Sangon Biotech Cat#C600387 Phosphatase inhibitor cocktail Sangon Biotech Cat#C500017 Native sample loading buffer Sangon Biotech Cat#C506032 DTT Sangon Biotech Cat#B645939 TRIzol Thermo Fisher Scientific Cat#15596018 M-MLV (H-) Reverse Transcriptase Vazyme Cat#R021-01 Act-D Sigma-Aldrich Cat#129935 Nonidet P-40 Yeasen Cat#20103ES60 Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 Magnetic beads carrying antibodies against Flag Bimake Cat#B26102 Magnetic beads carrying antibodies against HA Bimake Cat#B26202 40,6-diamidino-2-phenylindole (DAPI) Beyotime Cat#P0131 Matrigel Corning Cat#354230 Human IL6 MESGEN Cat#MIL1006 Critical commercial assays Enhanced Chemiluminescence (ECL) kit Fdbio science Cat#FB8020 Silver Staining Kit CWBIO Cat#CW2012 ChIP-IT Express Kit Active Motif Cat#53008 Human IL6 ELISA Kit Beyotime Cat#PI330 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Deposited data RNA-seq This paper GSE210093 Experimental models: Cell lines Human HBE cells Procell Life Science & Technology company Cat#CL-0346 Human BEAS-2B cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-5067 Human A549 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-503 Human H1650 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-592 Human H1299 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-589 Human embryonic kidney (HEK) 293T cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-502 Experimental models: Organisms/strains Mouse: female BALB/c nude mice the Laboratory Animal Center of Soochow University N/A (Continued on next page) Cell Reports 41, 111513, October 18, 2022 e2 Article ll OPEN ACCESS
Chromatin Immunoprecipitation:Article Title: TIF1γ inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BamHI New England Biolabs Cat#R0136L HindIII New England Biolabs Cat#R0104L EcoRI New England Biolabs Cat#R0101L XbaI New England Biolabs Cat#R0145L T4 DNA ligase buffer Thermo Fisher Scientific Cat#46300018 Lipofectamine 3000 Invitrogen Cat#L3000015 Puromycin Solarbio Cat#P8230 RIPA buffer Thermo Fisher Scientific Cat#89900 Protease inhibitor cocktail Sangon Biotech Cat#C600387 Phosphatase inhibitor cocktail Sangon Biotech Cat#C500017 Native sample loading buffer Sangon Biotech Cat#C506032 DTT Sangon Biotech Cat#B645939 TRIzol Thermo Fisher Scientific Cat#15596018 M-MLV (H-) Reverse Transcriptase Vazyme Cat#R021-01 Act-D Sigma-Aldrich Cat#129935 Nonidet P-40 Yeasen Cat#20103ES60 Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 Magnetic beads carrying antibodies against Flag Bimake Cat#B26102 Magnetic beads carrying antibodies against HA Bimake Cat#B26202 40,6-diamidino-2-phenylindole (DAPI) Beyotime Cat#P0131 Matrigel Corning Cat#354230 Human IL6 MESGEN Cat#MIL1006 Critical commercial assays Enhanced Chemiluminescence (ECL) kit Fdbio science Cat#FB8020 Silver Staining Kit CWBIO Cat#CW2012 ChIP-IT Express Kit Active Motif Cat#53008 Human IL6 ELISA Kit Beyotime Cat#PI330 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Deposited data RNA-seq This paper GSE210093 Experimental models: Cell lines Human HBE cells Procell Life Science & Technology company Cat#CL-0346 Human BEAS-2B cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-5067 Human A549 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-503 Human H1650 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-592 Human H1299 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-589 Human embryonic kidney (HEK) 293T cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-502 Experimental models: Organisms/strains Mouse: female BALB/c nude mice the Laboratory Animal Center of Soochow University N/A (Continued on next page) Cell Reports 41, 111513, October 18, 2022 e2 Article ll OPEN ACCESS
Enzyme-linked Immunosorbent Assay:Article Title: TIF1γ inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BamHI New England Biolabs Cat#R0136L HindIII New England Biolabs Cat#R0104L EcoRI New England Biolabs Cat#R0101L XbaI New England Biolabs Cat#R0145L T4 DNA ligase buffer Thermo Fisher Scientific Cat#46300018 Lipofectamine 3000 Invitrogen Cat#L3000015 Puromycin Solarbio Cat#P8230 RIPA buffer Thermo Fisher Scientific Cat#89900 Protease inhibitor cocktail Sangon Biotech Cat#C600387 Phosphatase inhibitor cocktail Sangon Biotech Cat#C500017 Native sample loading buffer Sangon Biotech Cat#C506032 DTT Sangon Biotech Cat#B645939 TRIzol Thermo Fisher Scientific Cat#15596018 M-MLV (H-) Reverse Transcriptase Vazyme Cat#R021-01 Act-D Sigma-Aldrich Cat#129935 Nonidet P-40 Yeasen Cat#20103ES60 Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 Magnetic beads carrying antibodies against Flag Bimake Cat#B26102 Magnetic beads carrying antibodies against HA Bimake Cat#B26202 40,6-diamidino-2-phenylindole (DAPI) Beyotime Cat#P0131 Matrigel Corning Cat#354230 Human IL6 MESGEN Cat#MIL1006 Critical commercial assays Enhanced Chemiluminescence (ECL) kit Fdbio science Cat#FB8020 Silver Staining Kit CWBIO Cat#CW2012 ChIP-IT Express Kit Active Motif Cat#53008 Human IL6 ELISA Kit Beyotime Cat#PI330 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Deposited data RNA-seq This paper GSE210093 Experimental models: Cell lines Human HBE cells Procell Life Science & Technology company Cat#CL-0346 Human BEAS-2B cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-5067 Human A549 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-503 Human H1650 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-592 Human H1299 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-589 Human embryonic kidney (HEK) 293T cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-502 Experimental models: Organisms/strains Mouse: female BALB/c nude mice the Laboratory Animal Center of Soochow University N/A (Continued on next page) Cell Reports 41, 111513, October 18, 2022 e2 Article ll OPEN ACCESS
Reporter Assay:Article Title: TIF1γ inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BamHI New England Biolabs Cat#R0136L HindIII New England Biolabs Cat#R0104L EcoRI New England Biolabs Cat#R0101L XbaI New England Biolabs Cat#R0145L T4 DNA ligase buffer Thermo Fisher Scientific Cat#46300018 Lipofectamine 3000 Invitrogen Cat#L3000015 Puromycin Solarbio Cat#P8230 RIPA buffer Thermo Fisher Scientific Cat#89900 Protease inhibitor cocktail Sangon Biotech Cat#C600387 Phosphatase inhibitor cocktail Sangon Biotech Cat#C500017 Native sample loading buffer Sangon Biotech Cat#C506032 DTT Sangon Biotech Cat#B645939 TRIzol Thermo Fisher Scientific Cat#15596018 M-MLV (H-) Reverse Transcriptase Vazyme Cat#R021-01 Act-D Sigma-Aldrich Cat#129935 Nonidet P-40 Yeasen Cat#20103ES60 Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 Magnetic beads carrying antibodies against Flag Bimake Cat#B26102 Magnetic beads carrying antibodies against HA Bimake Cat#B26202 40,6-diamidino-2-phenylindole (DAPI) Beyotime Cat#P0131 Matrigel Corning Cat#354230 Human IL6 MESGEN Cat#MIL1006 Critical commercial assays Enhanced Chemiluminescence (ECL) kit Fdbio science Cat#FB8020 Silver Staining Kit CWBIO Cat#CW2012 ChIP-IT Express Kit Active Motif Cat#53008 Human IL6 ELISA Kit Beyotime Cat#PI330 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Deposited data RNA-seq This paper GSE210093 Experimental models: Cell lines Human HBE cells Procell Life Science & Technology company Cat#CL-0346 Human BEAS-2B cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-5067 Human A549 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-503 Human H1650 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-592 Human H1299 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-589 Human embryonic kidney (HEK) 293T cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-502 Experimental models: Organisms/strains Mouse: female BALB/c nude mice the Laboratory Animal Center of Soochow University N/A (Continued on next page) Cell Reports 41, 111513, October 18, 2022 e2 Article ll OPEN ACCESS
RNA Sequencing:Article Title: TIF1γ inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BamHI New England Biolabs Cat#R0136L HindIII New England Biolabs Cat#R0104L EcoRI New England Biolabs Cat#R0101L XbaI New England Biolabs Cat#R0145L T4 DNA ligase buffer Thermo Fisher Scientific Cat#46300018 Lipofectamine 3000 Invitrogen Cat#L3000015 Puromycin Solarbio Cat#P8230 RIPA buffer Thermo Fisher Scientific Cat#89900 Protease inhibitor cocktail Sangon Biotech Cat#C600387 Phosphatase inhibitor cocktail Sangon Biotech Cat#C500017 Native sample loading buffer Sangon Biotech Cat#C506032 DTT Sangon Biotech Cat#B645939 TRIzol Thermo Fisher Scientific Cat#15596018 M-MLV (H-) Reverse Transcriptase Vazyme Cat#R021-01 Act-D Sigma-Aldrich Cat#129935 Nonidet P-40 Yeasen Cat#20103ES60 Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 Magnetic beads carrying antibodies against Flag Bimake Cat#B26102 Magnetic beads carrying antibodies against HA Bimake Cat#B26202 40,6-diamidino-2-phenylindole (DAPI) Beyotime Cat#P0131 Matrigel Corning Cat#354230 Human IL6 MESGEN Cat#MIL1006 Critical commercial assays Enhanced Chemiluminescence (ECL) kit Fdbio science Cat#FB8020 Silver Staining Kit CWBIO Cat#CW2012 ChIP-IT Express Kit Active Motif Cat#53008 Human IL6 ELISA Kit Beyotime Cat#PI330 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Deposited data RNA-seq This paper GSE210093 Experimental models: Cell lines Human HBE cells Procell Life Science & Technology company Cat#CL-0346 Human BEAS-2B cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-5067 Human A549 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-503 Human H1650 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-592 Human H1299 cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-589 Human embryonic kidney (HEK) 293T cells the Cell Bank of Chinese Academy of Sciences Cat#SCSP-502 Experimental models: Organisms/strains Mouse: female BALB/c nude mice the Laboratory Animal Center of Soochow University N/A (Continued on next page) Cell Reports 41, 111513, October 18, 2022 e2 Article ll OPEN ACCESS
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